Journal: Alzheimer's Research & Therapy

Article Title: CI-994 is a dual modulator of class I HDACs and Wnt/β-catenin signaling for the treatment of Alzheimer's disease

doi: 10.1186/s13195-026-01982-0

Figure Lengend Snippet: Activation of Wnt/β-catenin signaling is associated with CI-994-mediated tau phosphorylation inhibition in patient-specific iPSC-derived cerebral organoids. A Wnt reporter assay in Wnt3A-expressing HEK293 cells with the treatment of CI-994 and LGK974 at indicated concentrations for 24 h. B - H The human cerebral organoids carrying APOE ε4/ε4 at 2 months of age cultured in 6-well plates (3 organoids per well) were treated in triplicate with CI-994 (8 µM) and LGK974 (200 nM) for 72 h. B - D The levels of acetyl histone H3K9, total histone H3, total β-catenin, β-actin, and α-tubulin were examined by Western blotting and quantified. E – H The levels of tau phosphorylation (AT8), total tau (HT7), β3-tubulin, and β-actin were examined by Western blotting and quantified. All the values are mean ± SEM. P values were calculated using one-way ANOVA with Dunnett’s multiple comparison test

Article Snippet: CI-994 (Selleck Chemicals LLC, cat. no. S2818), niclosamide (Selleck Chemicals LLC, cat. no. S3030), Wnt agonist BML-284 (Selleck Chemicals LLC, cat. no. 8178), Wnt activator LGK974 (Selleck Chemicals LLC, cat. no. 7143), and HDAC activator ITSA-1 (Selleck Chemicals LLC, cat. no. S8323) were dissolved in dimethyl sulfoxide (DMSO) at 20—100 mM and stored at −20 °C.

Techniques: Activation Assay, Phospho-proteomics, Inhibition, Derivative Assay, Reporter Assay, Expressing, Cell Culture, Western Blot, Comparison